Then the bed continues to expand smoothly until some of the gas begins to disengage from the homogeneous dense phase and forms bubbles. Results: the chromatogram obtained with the test solution shows zones corresponding to those in the chromatograms obtained with reference solutions (a) and (b), but shows no zones corresponding to those in the chromatograms obtained with reference solutions (c) and (d); the chromatogram obtained with the test solution also shows a zone with a very low RF value (2,4-diaminobutyric acid).
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